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H2DCFDA試劑

參考價	￥250.00-￥700.00

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公司名稱杭州昊鑫生物科技股份有限公司
品牌MCE
型號HY-D0940
所在地杭州市
廠商性質代理商
更新時間2023/7/1 10:40:36
訪問次數(shù)730

產(chǎn)品標簽:

DCFH-DA 2'7'-Dichlorodihydrofluorescein diacetate H2DCFDA

規(guī)格

10 mM * 1 mL in DMSO	550.00元	10000 支可售
10 mg	250.00元	10000 支可售
25 mg	370.00元	10000 支可售
50 mg	500.00元	10000 支可售
100 mg	700.00元	10000 支可售

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杭州昊鑫生物科技股份有限公司成立于2009年初，是一家面向生命科學領域，從事科研機構、高校、院所及生產(chǎn)企業(yè)所需科研試劑、耗材，儀器銷售和服務面向全國。

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產(chǎn)品簡介在線詢價

產(chǎn)地	進口	級別	其他

H2DCFDA試劑
H2DCFDA (DCFH-DA) 是一種可滲透細胞的，用于檢測細胞內活性氧 (ROS) 的探針 (Ex/Em=488/525 nm)。

H2DCFDA試劑產(chǎn)品信息

H2DCFDA試劑

H2DCFDA (DCFH-DA) 是一種可滲透細胞的，用于檢測細胞內活性氧 (ROS) 的探針 (Ex/Em=488/525 nm)。

H2DCFDA試劑生物活性

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)

分子量

487.29

性狀

Solid

Formula

C₂₄H₁₆Cl₂O₇

CAS 號

4091-99-0

Emission (Em)

517

Excitation (Ex)

492

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)

In Vitro:

DMSO : 125 mg/mL (256.52 mM; Need ultrasonic)

Ethanol : 20 mg/mL (41.04 mM; Need ultrasonic)

配制儲備液

濃度溶劑體積質量	1 mg	5 mg	10 mg

1 mM	2.0522 mL	10.2608 mL	20.5217 mL
5 mM	0.4104 mL	2.0522 mL	4.1043 mL
10 mM	0.2052 mL	1.0261 mL	2.0522 mL

請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲存時，請在 6 個月內使用，-20°C 儲存時，請在 1 個月內使用。

In Vivo:

請根據(jù)您的實驗動物和給藥方式選擇適當?shù)娜芙夥桨?。以下溶解方案都請先按?nbsp;In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：

——為保證實驗結果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

1.

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.08 mg/mL (4.27 mM); Suspended solution; Need ultrasonic
2.

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution
3.

請依序添加每種溶劑： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

染色示例	Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Stain cells with H2DCFDA. 2. Capture staining cells by spinning-disk confocal microscopy (every 5 min for 6 h). Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Remove the cell culture medium and replaced by fresh medium containing H2DCFDA and incubated with cultured cells for 30 min. 2. Wash cells with PBS for three times. 3. Use a confocal laser scanning microscopy (FV1000, Olympus Company, Japan) to detect the intracellular ROS generation. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Testing cells are seeded into a 6-well microplate at a density of 10⁵/well and cultured overnight at 37°C in 5% CO₂. Fresh medium with 50 μg/mL of different materials is added to each well and cultured for 16 h. 2. Wash cells with PBS and stain cells with H2DCFDA (20 μM; 1 h; 37°C; dark). 3. Cells are trypsinized, washed, resuspended in PBS (0.2 mL), analyzed by a flow cytometer and use a confocal laser scanning microscopy (FV1000, Olympus, Tokyo, Japan) for image. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining. 1. Wash cultured cells with PBS for three times. 2. Treat testing cells with H2DCFDA in culture medium for 0.5 h in a cell incubator. 3. Use a confocal laser scanning microscopy (Olympus, FV3000) for image. Cells are analyzed on a BD LSRI Fortessa flow cytometer analyzer to quantify results. Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence. Method: For cell staining.

染色示例

Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Stain cells with H2DCFDA.
2. Capture staining cells by spinning-disk confocal microscopy (every 5 min for 6 h).
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Remove the cell culture medium and replaced by fresh medium containing H2DCFDA and incubated with cultured cells for 30 min.
2. Wash cells with PBS for three times.
3. Use a confocal laser scanning microscopy (FV1000, Olympus Company, Japan) to detect the intracellular ROS generation.
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Testing cells are seeded into a 6-well microplate at a density of 10⁵/well and cultured overnight at 37°C in 5% CO₂. Fresh medium with 50 μg/mL of different materials is added to each well and cultured for 16 h.
2. Wash cells with PBS and stain cells with H2DCFDA (20 μM; 1 h; 37°C; dark).
3. Cells are trypsinized, washed, resuspended in PBS (0.2 mL), analyzed by a flow cytometer and use a confocal laser scanning microscopy (FV1000, Olympus, Tokyo, Japan) for image.
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.
1. Wash cultured cells with PBS for three times.
2. Treat testing cells with H2DCFDA in culture medium for 0.5 h in a cell incubator.
3. Use a confocal laser scanning microscopy (Olympus, FV3000) for image. Cells are analyzed on a BD LSRI Fortessa flow cytometer analyzer to quantify results.
Description: H2DCFDA (DCFH-DA) is a ROS production probe with green fluorescence.
Method: For cell staining.