抗磷脂抗體（AplAPA）ELISA檢測(cè)試劑盒

抗磷脂抗體（AplAPA）ELISA檢測(cè)試劑盒詳細(xì)說(shuō)明書(shū)請(qǐng)來(lái)電，或者在線客服進(jìn)行索要！齊一生物長(zhǎng)期經(jīng)營(yíng)ELISA試劑盒及抗體（免費(fèi)代測(cè)）、分子學(xué)生物試劑盒、比色法，IFCC推薦法，化學(xué)氧化法，速率法等檢測(cè)試劑盒、細(xì)胞菌株、培養(yǎng)基、細(xì)胞因子、生化試劑、耗材等生物試劑產(chǎn)品。誠(chéng)信經(jīng)營(yíng)，價(jià)格實(shí)惠，服務(wù)周到，質(zhì)量保障！提供各種實(shí)驗(yàn)代測(cè)服務(wù)。歡迎新老客戶！

抗磷脂抗體（AplAPA）ELISA檢測(cè)試劑盒產(chǎn)品規(guī)格：96T/48T
產(chǎn)品庫(kù)存：現(xiàn)貨
產(chǎn)品價(jià)格：詢價(jià)
使用范圍：僅供科研檢測(cè),不得用于臨床.
保存條件：2-8℃
發(fā)貨方式：專門(mén)快遞免費(fèi)送貨上門(mén)
產(chǎn)品價(jià)格：*,洽談！
說(shuō)明書(shū):說(shuō)明書(shū)隨貨發(fā)送,您也可以直接我司在線銷售人員索取.全國(guó)：代理多種品牌進(jìn)口原裝、分裝“ELISA試劑盒”.齊一生物科技有限公司作為酶聯(lián)免疫供應(yīng)商,憑借*的技術(shù),*的售前,售中,售后免費(fèi)代測(cè)服務(wù),共同鑄就高品質(zhì)的產(chǎn)品.多年專業(yè)酶免服務(wù),質(zhì)量保證,可免費(fèi)提供代測(cè)服務(wù),一周內(nèi)出結(jié)果.歡迎訂購(gòu).
ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產(chǎn)生大量的泡沫,以免加樣時(shí)加入大量的氣泡,產(chǎn)生加樣上的誤差.
根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù).每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔.每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定,能使用復(fù)孔的盡量做復(fù)孔.標(biāo)本用標(biāo)本稀釋液1：1稀釋后加入50ul于反應(yīng)孔內(nèi).
加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi).立即加入50ul的生物素標(biāo)記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時(shí).
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內(nèi)液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復(fù)此操作3次.如果用洗板機(jī)洗滌,洗滌次數(shù)增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標(biāo)板,迅速加入50ul終止液,加入終止液后應(yīng)立即測(cè)定結(jié)果.
在450nm波長(zhǎng)處測(cè)定各孔的OD值.
ELISA操作步驟
酶聯(lián)免疫吸附實(shí)驗(yàn)材料,試劑,溶液
1．酶標(biāo)板,100μltip頭,1mlEp管,濕盒
2．包被稀釋液（0.05mol/L碳酸鈉-碳酸氫鈉buffer,pH9.6）
碳酸鈉0.15g,碳酸氫鈉0.29g,疊氮鈉0.02g,加雙蒸水100ml,調(diào)pH9.6
3.封閉液(5%小牛血清/PBS溶液)
小牛血清5ml,1*PBS(pH7.4)95ml
4.洗滌液(PBST,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,Tween200.05ml,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
5.樣本稀釋液(PBS,pH7.4)
NaCl0.8g,KH2PO40.02g,Na2HPO4.12H2O0.29g,KCl0.02g,疊氮鈉0.01g,加雙蒸水100ml,調(diào)pH7.4
6.酶標(biāo)第2抗體(羊抗兔)稀釋范圍1:5,000-1:100,000
7.底物液(TMB-過(guò)氧化氫尿素溶液)
①底物液A:TMB20mg,無(wú)水乙醇10ml,加雙蒸水100ml.
②底物液B(0.1mol/L檸檬酸-0.2mol/L磷酸二氫鈉緩沖液,pH5.0-5.4)
Na2HPO41.46g,檸檬酸0.933g,0.75%過(guò)氧化氫尿素0.64ml,加三蒸水100ml,調(diào)pH5.0-5.4
③底物A和B按1:1混合即成TMB-過(guò)氧化氫尿素溶液.
8.終止液(2mol/LH2SO4溶液)雙蒸水200ml,濃硫酸34ml,(緩慢滴加并不斷攪拌),加雙蒸水300ml
9.0.9%生理鹽水

800356    Handling Fee
9015298    Nozzles, M48 (6)
9243775    QIAsymphony AS Custom Protocol Advanced2
9019447    Sensor brd 4 CH temp sensor
9019444    Electrolytic capacitor 8000uF x 63V
9018158    Waste station, waste drawer, Qsym
9018154    Bottle, waste drawer, Qsym
9018135    Sensor, xz, et/hs, Qsym
9018132    Splash guard, extractor, Qsym
9017720    Appl. Pack Investigator for Univ.PF
9017832    Sensor, tip check, QIAC
9017830    Power entry port, QIAC
9017828    Power supply, 230 V, QIAC
9017821    Board, main, QIAC
9017820    Flexcircuit, display, 40 pol. , QIAC
9017812    Board, centrifuge (240V), QIAC
4846024    careUU PCR ASSAY
4861024    careEBV PCR ASSAY II
130906    NeXtal DWBlock MPD Suite
4518265    artus HCV RG RT-PCR Kit (96) CE
130720    NeXtal Tubes JCSG+ Suite
129114    Nuclease-Free Water (10 x 50 ml)
4504065    artus Parvo B19 LC PCR Kit (96) CE
120025    TurboFilter 96 Plates (96)    96 TurboFilter 96 Plates
4505065    artus HAV LC RT-PCR Kit (96) CE
4506063    artus HBV LC PCR Kit (24) CE
75742    RNeasy Fibrous Tissue Midi Kit (10)    10 RNeasy Midi Spin Columns, Collection Tubes (15 ml), Proteinase K, RNase-free DNase I, RNase-free Reagents and Buffers
74181    RNeasy 96 Kit (4)    For 4 x 96 total RNA preps: 4 RNeasy 96 Plates, Elution Microtubes CL (1.2 ml), Caps, RNase-free Reagents and Buffers
28053    MinElute 96 UF PCR Purification Kit (24)    24 MinElute 96 UF PCR Purification Plates
74121    RNeasy Protect Starter Kit    RNAlater RNA Stabilization Reagent (20 ml), 6 RNeasy Mini Spin Columns, 2 RNeasy Midi Spin Columns, Collection Tubes (1.5 and 2 ml), RNase-free Reagents and Buffers
74104    RNeasy Mini Kit (50)    50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
72251    TurboCapture 96 mRNA Kit (5)    For rapid and easy mRNA purification in high-throughput applications. The kit provides a fast and simple procedure for purifying mRNA. Up to 96 samples can be processed simultaneously - ideal for high-throughput applications such as pound screening and target validation (e.g. by siRNA screening).
19407    VacConnectors (500)    500 disposable connectors for use with QIAamp spin columns on luer connectors. Should be ordered in conjuntion with 19408 - VacValves (24).
12281    QIAfilter Plasmid Mega Kit (5)    5 QIAGEN-tip 2500, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
12191    QIAGEN Plasmid Giga Kit (5)    5 QIAGEN-tip 10000, Reagents, Buffers
4502103    artus VZV TM PCR Kit (24) RUO
133271    NeXtalStock PIPES pH 7.0 (200)
133270    NeXtalStock Phosphate-citr. pH 5.2 (200)
990512    Filter-Tips, 50 μl (960)
337620    qBiomarker SYBR Green Fluor Mastermix(2)
337011    qBiomarker Somatic Mutations PCR Assays
4500263    artus HSV-1/2 RG PCR Kit (24) CE
939016    Buffer ATL ( 4 x 50 ml),GPR
4500163    artus HSV-1/2 TM PCR Kit (24) CE
1073925    Ni-NTA Superflow (8 x 500 ml),cus,G
9010931    BGR Greifer P7 (alt)
9010858    BGR DT-Abstreifer 8-fach P7xxx D
931036    QIAsymphony Virus/Bact Mini Kit (192)
133248    NeXtalStock Sodium succinate pH6.5 (200)
330603    RT2 SYBR Green FAST Mastermix (24)
929608    QX Pro Separation Buffer (40 ml)
5000-1415    Knob RB671CSKWL Matte, VTX2
5000-1408    Cable Shielded 1/17HP, VTX2
L2170    hc2 CT/GC HC2 DNA Test PI (CD-ROM)
L1971    Rotary Shaker User's Manual
9240705    O-ring Kit, Capper, Spindle, AP
9240693    Actril Sterilant & Test Strips, AP
9243653    Comprehen Valid., Other Extraction Sys    """The Comprehensive Validation Support plan specific to a laboratory's needs will incorporate all of the requirements for the internal validation guidelines from the FBI's Quality Assurance Standards (QAS).

The Comprehensive Validation Support service includes all travel and labor charges for up to 20 days by the QIAGEN Validation Team. Typically, one week will be required to extract, quantify, and amplify the validation study samples and may require multiple individuals. Usually, two weeks is required to analyze the data and one week to write the final report. The expected time period to plete the Comprehensive Validation is six weeks. The actual time may vary depending on resource availability, including laboratory time dedicated to validation activities.

Lab Work – The QIAGEN specialist(s) will travel to the laboratory to conduct the on-site validation lab work. The QIAGEN specialist team will perform and plete all extractions, quantifications, amplfications, and capillary electrophoresis. The laboratory is responsible for providing systems/protocols that may be needed (generally laboratory SOPs, ex. interpretation guidelines). Additionally, the laboratory staff may be required to provide additional data needed for interpretation (ex. quantification/amplification/CE results) during the data review phase of the validation. 

Once the final scope of the validation is determined, QIAGEN will provide a quote for all consumables required for the validation.  Non-QIAGEN chemistry and accessories are not included and should  be purchased to cover the validation experimental design including qPCR, STR, NIST standards, required samples types (blood, semen, buccal samples) and required substrates (swabs, materials, etc).   The Customer is expected to have all materials on-hand 1 week prior to the validation start date.

Data Analysis – The Comprehensive Validation Support plan enpasses data collection (quants, amps, CE) during the on-site visit. The laboratory may be responsible for sharing (typically electronically via ) any validation data requested after the on-site visit. A QIAGEN specialist will plete all of the data analysis produced from the validation study design. This includes qPCR (quant) and STR (amp) analysis and linkage to starting samples.

Validation data CD/flashdrive – QIAGEN will review the validation data and draft a validation summary document, ensuring pliance with the FBI's QAS requirements/standards. QIAGEN will send validation data in an electronic format (/CD/flashdrive) to the laboratory. The laboratory is responsible for final pilation, any format change preferences, review and signoff of the validation report. The documentation provided by QIAGEN will aide in demonstrating pliance with all accreditation guidelines during laboratory audits. Should a hard copy of the validation study be preferred, the laboratory is required to specify prior to the onset of the validation.

Post-validation QIAGEN-to-Laboratory Transfer – Following pletion of the validation and generation of the draft validation summary document, a designated member of the QIAGEN validation team will review the validation with the Lab Manager and the Technical Leader. Following sign off on the validation report, training of the laboratory DNA staff will occur. Refer to the Post-validation Competency Training for additional information. """
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