大鼠凝溶膠蛋白(Gelsolin)ELISA 檢測試劑盒,大鼠凝溶膠蛋白(Gelsolin)ELISA 檢測試劑盒
大鼠凝溶膠蛋白(Gelsolin)ELISA 檢測試劑盒*試驗原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進行檢測。先將ZR和生物素標(biāo)記的抗體同時溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
大鼠凝溶膠蛋白(Gelsolin)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12634B 雞主要組織相容性復(fù)合體(MHC/B)elisa試劑盒 Chicken major histocompatibility complex,MHC/B Elisa試劑盒
RS12635B 雞熱休克蛋白70(HSP-70)elisa試劑盒 Chicken Heat Shock Protein 70,Hsp-70 Elisa試劑盒
RS12636B 雞白痢抗體檢測(PD)elisa試劑盒 Chiken Pullorum Diseaes,PD Elisa試劑盒
RS12637B 雞分泌型免疫球蛋白A(SIgA)elisa試劑盒 Chicken secretory immunoglobulin A,SIgA Elisa試劑盒
RS12638B 雞α干擾素(IFN-α)elisa試劑盒 Chicken Interferon α,IFN-α Elisa試劑盒
RS12639B 雞白介素18(IL-18)elisa試劑盒 Chicken Interleukin 18,IL-18 Elisa試劑盒
RS12640B 雞白介素1(IL-1)elisa試劑盒 Chicken Interleukin 1,IL-1 Elisa試劑盒
RS12641B 雞病毒性腸炎病毒(DEV)elisa試劑盒 Chicken virus enteritis,DEV Elisa試劑盒
RS12642B 雞肌紅蛋白(MYO/MB) elisa試劑盒 Chicken Myoglobin,MYO/MB Elisa試劑盒
RS12643B 雞白介素9(IL-9)elisa試劑盒 Chicken Interleukin 9,IL-9 Elisa試劑盒
RS12644B 雞白介素3(IL-3)elisa試劑盒 Chicken Interleukin 3,IL-3 Elisa試劑盒
RS12645B 雞白血病抑制因子(LIF)elisa試劑盒 Chicken Leukemia inhibitory factor,LIF Elisa試劑盒
RS12646B 雞轉(zhuǎn)化生長因子β1(TGF-β1)elisa試劑盒 Chicken Transforming Growth factor β1,TGF-β1 Elisa試劑盒
RS12647B 雞C反應(yīng)蛋白(CRP)elisa試劑盒 Chicken C-Reactive Protein,CRP Elisa試劑盒
RS12648B 雞血管內(nèi)皮細(xì)胞生長因子(VEGF)elisa試劑盒 Chicken Vascular Endothelial cell Growth Factor,VEGF Elisa試劑盒
RS12649B 雞免疫球蛋白G(IgG)elisa試劑盒 Chicken Immunoglobulin G,IgG Elisa試劑盒
RS12650B 雞堿性成纖維細(xì)胞生長因子(bFGF)elisa試劑盒 Chicken basic fibroblast growth factor,bFGF Elisa試劑盒
RS12651B 雞可溶性細(xì)胞間粘附分子1(sICAM-1)elisa試劑盒 Chicken Soluble InteFAellular Adhesion Molecule-1,sICAM-1 Elisa試劑盒
RS12652B 雞可溶性E選擇素(sE-selectin)elisa試劑盒 Chicken soluble E-selectin,sE-selectin Elisa試劑盒
RS12653B 雞表皮生長因子(EGF)elisa試劑盒 Chicken Epidermal growth factor,EGF Elisa試劑盒
RS12654B 雞胰島素樣生長因子1(IGF-1)elisa試劑盒 Chicken Insulin-like growth factors 1,IGF-1 Elisa試劑盒
RS12655B 雞生長因子(GH)elisa試劑盒 Chicken growth hormone,GH Elisa試劑盒
RS12656B 雞乙酰乙酸檢測(ACAC)elisa試劑盒 Chicken Acetoacetic acid,ACAC Elisa試劑盒 ab25799 Rat monoclonal [20d5] to NKG2A + C+ E (Phycoerythrin) 100tests 3450
ab258 Mouse monoclonal [4C6-H6] to TNF alpha 2ml 3450
ab25800 Rabbit polyclonal to CSN7b 100ug 3450
ab25802 Mouse monoclonal [192] to CA19-9 100ug 3450
ab25803 Mouse monoclonal [S8] to MCP1 100ug 3450
ab25804 Mouse monoclonal [10C12] to CD4 100ug 3450
ab25805 Rabbit polyclonal to AKAP9 50ug 3450
ab25806 Goat polyclonal to HEK293 Host Cell Proteins 100ug 3450
ab25807 Goat polyclonal to MRC5 Host Cell Proteins 100ug 3450
ab25808 Guinea pig polyclonal to Neisseria meningitidis 100ul 3450
ab25809 Goat polyclonal to NS/0 Host Cell Proteins 100ug 3450
ab2581 Mouse monoclonal [1B2] to HIV1 p55 + HIV1 p17 100ug 3450
ab25810 Goat polyclonal to P.pastoris 100ug 3450
ab25811 Rabbit polyclonal to Lactoferrin (Biotin) 100ul 3450
ab25812 Goat polyclonal to Pseudomonas fluorescens 200ug 3450
ab25813 Goat polyclonal to Saccharomyces cerevisiae 100ug 3450
ab25815 Goat polyclonal to SP2/0 Host Cell Proteins 100ug 3450
ab25816 Goat polyclonal to A549 100ug 3450
ab25819 Rabbit polyclonal to Cullin 3, prediluted 7ml 3450
ab2582 Mouse monoclonal [1E5] to Hepatitis C Virus Core Antigen 100ug 3450
ab25820 Mouse monoclonal [SPM264] to Cytokeratin 16 7ml 3450
ab25823 Goat polyclonal to E.coli 100ug 3450
ab25824 Rabbit polyclonal to Total Goat Milk Proteins 100ug 3450
ab25825 Mouse monoclonal [38] to CD11a (Biotin) 100ug 3450
ab25827 Mouse monoclonal [44] to CD11b (FITC) 100ug 3450
ab25828 Rat monoclonal [TM38] to Vimentin 100ug 3450
ab2583 Mouse monoclonal [1E5] to Hepatitis C Virus Core Antigen (Biotin) 50ug 3450
ab25830 Mouse monoclonal [4A5] to MEK1 100ug 3450
ab25831 Rabbit polyclonal to AP2 alpha 100ug 3450
ab25832 Mouse monoclonal [FPS392] to p53 (phospho S392) 50ug 3450
ab25833 Mouse monoclonal [VM64] to CD31 100ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實驗中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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