大鼠硫氧化還原蛋白(Trx)ELISA 檢測試劑盒,大鼠硫氧化還原蛋白(Trx)ELISA 檢測試劑盒
大鼠硫氧化還原蛋白(Trx)ELISA 檢測試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
大鼠硫氧化還原蛋白(Trx)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
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RS12670B 雞熱休克蛋白60(Hsp-60)elisa試劑盒 Chicken Heat Shock Protein 60,HSP-60 Elisa試劑盒
RS12671B 雞白介素4(IL-4)elisa試劑盒 Chicken Interleukin 4,IL-4 Elisa試劑盒
RS12672B 雞白介素2(IL-2)elisa試劑盒 Chicken Interleukin 2,IL-2 Elisa試劑盒
RS12673B 雞血清一氧化氮(NO)elisa試劑盒 Chicken nitric oxide,NO Elisa試劑盒
RS12674B 雞單純皰疹病毒Ⅱ型抗體(HSVⅡ-Ab)elisa試劑盒 Chicken Herpes simplexvirus Ⅱ antibody,HSVⅡ-Ab Elisa試劑盒
RS12675B 雞補(bǔ)體3裂解產(chǎn)物(C3SP)elisa試劑盒 Chicken Complement 3 split product,C3SP Elisa試劑盒
RS12676B 雞腎上腺髓質(zhì)素(ADM)elisa試劑盒 Chicken adrenomedullin,ADM Elisa試劑盒
RS12677B 雞巨噬細(xì)胞替代激活相關(guān)化學(xué)因子1(AmAC-1)elisa試劑盒 Chicken Alternative macrophage activation-associated CC chemokine 1,AmAC-1 Elisa試劑盒
RS12678B 雞免疫核糖核酸(Irna)elisa試劑盒 Chicken Immune RNA,Irna Elisa試劑盒
RS12679B 雞β內(nèi)酰胺酶(β-lactamase)elisa試劑盒 Chicken β-lactamase Elisa試劑盒
RS12680B 雞流行性乙型腦炎抗體IgG(JE IgG)elisa試劑盒 Chicken Japanese Encephalitis IgG,JE IgG Elisa試劑盒
RS12681B 雞α葡萄糖苷酶(α-glucosidase)elisa試劑盒 Chicken α-Glucosidase Elisa試劑盒
RS12682B 雞血小板因子4(PF-4/CXCL4)elisa試劑盒 Chicken Plaet Factor 4,PF-4 Elisa試劑盒
RS12683B 雞腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體3(TRAIL-R3)elisa試劑盒 Chicken tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3 Elisa試劑盒ab14000 Chicken polyclonal to BCAS2 50ug 3450
ab14001 Chicken polyclonal to beta Actin 50ug 3450
ab14002 Chicken polyclonal to BirA 50ug 3450
ab14003 Chicken polyclonal to BRCA1 50ug 3450
ab14004 Chicken polyclonal to C1q 50ug 3450
ab14006 Chicken polyclonal to CD14 50ug 3450
ab14007 Chicken polyclonal to CD34 50ug 3450
ab14008 Chicken polyclonal to C Reactive Protein 50ug 3450
ab14009 Chicken polyclonal to CRIP2 50ug 3450
ab1401 Rabbit polyclonal to NPFF1 Receptor 50ug 3450
ab14010 Chicken polyclonal to Neuroglobin 50ug 3450
ab14011 Chicken polyclonal to CSRP2 50ug 3450
ab14013 Chicken polyclonal to CSRP3 50ug 3450
ab14014 Chicken polyclonal to Dnmt3b 50ug 3450
ab14015 Chicken polyclonal to E Cadherin 50ug 3450
ab14016 Chicken polyclonal to EPO 50ug 3450
ab14017 Chicken polyclonal to EGFR 50ug 3450
ab14018 Chicken polyclonal to ERK2 50ug 3450
ab14019 Chicken polyclonal to ERK3 50ug 3450
ab1402 Rabbit polyclonal to NPFF2 Receptor 50ug 3450
ab14020 Chicken polyclonal to Estrogen Receptor alpha 50ug 3450
ab14021 Chicken polyclonal to Estrogen Receptor beta 50ug 3450
ab14022 Chicken polyclonal to CD95 50ug 3450
ab14023 Chicken polyclonal to GALK1 50ug 3450
ab14024 Chicken polyclonal to GM-CSF 50ug 3450
ab14025 Rabbit polyclonal to Pleiotrophin 50ug 3450
ab14026 Chicken polyclonal to GRO alpha 50ug 3450
ab14027 Chicken polyclonal to ErbB 2 50ug 3450
ab14028 Chicken polyclonal to Histone H1 50ug 3450
ab14029 Chicken polyclonal to HIV1 tat 50ug 3450
ab1403 Rabbit polyclonal to Prostein 50ug 3450
ab14030 Chicken polyclonal to IgE 50ug 3450
ab14032 Chicken polyclonal to IGF1 Receptor 50ug 3450
ab14033 Chicken polyclonal to Mouse IgG 50ug 3450
ab14034 Chicken polyclonal to IKK beta 50ug 3450
ab14035 Chicken polyclonal to IL16 50ug 3450
ab14036 Chicken polyclonal to IL2 50ug 3450
ab14037 Chicken polyclonal to IL21 50ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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