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C-反應蛋白抗體Anti-CRP齊一生物科技有限公司主要經(jīng)營進口原裝Elisa試劑盒,種類齊全,價格實惠,貨期短。購買我公司ELISA產(chǎn)品客戶,可享免費代測、全程技術(shù)指導:(包括售前標本收集、后期實驗、實驗結(jié)束后數(shù)據(jù)分析等技術(shù)問題,我公司會有專業(yè)的技術(shù)即時給您去電溝通、解決。)保證售后服務。
C-反應蛋白抗體Anti-CRP相關(guān)標記 Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 647 AP APC Biotin Cy3 Cy5
Cy5.5 Cy7 FITC Gold HRP PE PE-Cy3 PE-CY5 PE-CY5.5 PE-CY7 RBITC
英文名稱 CRP
中文名稱 C-反應蛋白抗體
研究領域 心血管 免疫學 信號轉(zhuǎn)導 細胞凋亡 生長因子和激素
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Pig, Rabbit,
產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500(石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 25kDa
細胞定位 分泌型蛋白
性 狀 Lyophilized or Liquid
濃 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CRP (151-200aa)
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
產(chǎn)品介紹 background:
Pentraxins are plasma proteins characterized by their pentameric discoid assembly and their Ca2+ dependent ligand binding, such as Serum amyloid P ponent (SAP) and C-reactive Protein (CRP), which are cytokine-inducible acute-phase proteins implicated in innate immunity. CRP binds to ligands containing phosphocholine, SAP binds to amyloid fibrils, DNA, chromatin, fibronectin, C4-binding proteins and glycosaminoglycans. "Long" pentraxins have N-terminal extensions to the mon pentraxin domain; one group, the neuronal pentraxins, may be involved in synapse formation and remodeling, and they may also be able to form heteromultimers.
Function:
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and plement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Subunit:
Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.
Subcellular Location:
Secreted.
Tissue Specificity:
Found in plasma.
Similarity:
Belongs to the pentaxin family.
Contains 1 pentaxin domain.
Gene ID:
1401
Database links:
Entrez Gene: 1401 Human
Omim: 123260 Human
SwissProt: P02741 Human
Unigene: 76452 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
C反應蛋白 CRP(C-reactive protin)是與C性肺炎球菌的多糖起反應的一種蛋白,在某些細菌的感染過程中出現(xiàn)在血漿中。
CRP是在一些病理性情況下出現(xiàn)于病人血清中的一種糖蛋白,特別是在伴有肯炎癥和組織壞死疾病的急性期。血清中出現(xiàn)的這種異常蛋白能和肺炎C-多糖體發(fā)生沉淀反應,當疾病好轉(zhuǎn)或緩解時,CRP減低或消失。
臨床意義:
(1)各種急性化膿性炎癥、菌血癥、組織壞死(心肌梗塞、手術(shù)、嚴重創(chuàng)傷、燒傷等)、惡性腫瘤、結(jié)締組織病、腎移殖后急性排異反應、急性風濕熱、風濕性關(guān)節(jié)炎、類風濕性關(guān)節(jié)炎、心肌炎急性期、肺炎、肺結(jié)核、系統(tǒng)性紅斑狼瘡、急性肝炎和闌尾炎等,血清中CRP含量可增高,它的出現(xiàn)比其它急性期的反應物質(zhì)早,所以對疾病的早期診斷很有幫助。
CRP可以鑒別細菌炎癥或者是病毒性炎癥。細菌性炎癥CRP可以明顯增高,而病毒性炎癥CRP的血清含量一般都在正常范圍。
產(chǎn)品圖片
Antigen: bs-0155P, 0.2ug/100ul
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat-Anti- Rabbit IgG(bs-0295G-HRP) at 1: 5000;
TMB(C-0024) staining;
Read the data in MicroplateReader by 450nm.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(bs-0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRP Polyclonal Antibody, Unconjugated(bs-0155R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining