說明書下載E2HL.rar

分類名稱 : 血液尿液化學(xué),代謝產(chǎn)物分析 產(chǎn)品介紹 :

測定方法     比色法（570nm）或熒光法（530nm/590nm）定量測定高低密度(HDL/LDL/VLDL)的含量。

產(chǎn)品說明     高密度脂蛋白（HDL）和低密度（LDL）/極低密度（VLDL）脂蛋白中的膽固醇濃度是冠心病強(qiáng)有力的預(yù)測證明，功能性HDL為清除細(xì)胞和動脈中 的膽固醇提供保護(hù)。由于動脈粥樣硬化的風(fēng)險性高，低密度脂蛋白中的高濃度膽固醇和高密度脂蛋白中的低濃度膽固醇與心血管疾病有著緊密的。高或者低密度 脂蛋白之間的平衡*由遺傳因素決定。但是能通過藥物治療，食物選擇和其他因素而得到改變。對于測量HDL和LDL/VLDL中膽固醇濃度。簡單﹑直接和自動化檢測手段是十分理想的，博世生物技術(shù)的HDL和LDL/VLDL 定量測試盒是基于經(jīng)過改良的HDL和LDL/VLDL測定方法。在570nm 處反應(yīng)產(chǎn)物的吸光強(qiáng)度或者在λem/ex = 585/530nm處的熒光強(qiáng)度直接與樣品中膽固醇濃度成正比。

產(chǎn)品特點     靈敏準(zhǔn)確：線性檢測范圍在96孔板，比色法1-100毫克/分升和熒光法0.2-10毫克/分升膽固醇。方便：室溫檢測，無需37攝氏度加熱器。

產(chǎn)品用途     直接測試：在血清中的HDL和LDL/VLDL膽固醇。 藥理研究：藥物對膽固醇代謝的影響。

貯存條件     室溫貯存PBS和沉淀試劑，其余試劑在-20攝氏度溫度下貯存。

DESCRIPTION  CHOLESTEROL concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. Our HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample.

APPLICATIONS  Direct Assays: HDL and LDL/VLDL cholesterol in serum samples. Pharmacology: evaluation of drugs on cholesterol metabolism.

KEY FEATURES  Sensitive and accurate. Linear detection range in 96-well plate: 1-100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed.

STORAGE CONDITIONS  Store PBS and Precipitation Reagent at room temperature and the rest reagents at -20°C. Shelf life of at least 6 months.

編號：E2HL-048, E2HL-100

測試：48T, 100T