低氧細(xì)胞工作站

HypoxyLab™是一款結(jié)構(gòu)緊湊，HEPA過濾，精密控制的低氧環(huán)境的工作站。

HypoxyLab為各種以細(xì)胞為基礎(chǔ)的研究領(lǐng)域創(chuàng)造適合的、無污染的低氧條件。

應(yīng)用領(lǐng)域包括腫瘤生物學(xué)，放射細(xì)胞生物學(xué)，心血管研究，細(xì)胞凋亡，神經(jīng)科學(xué)，干細(xì)胞研究，多學(xué)科的藥物開發(fā)和蛋白質(zhì)組學(xué)。

真正的低氧復(fù)制

HypoxyLab™是調(diào)節(jié)培養(yǎng)環(huán)境中氧氣分壓的低氧工作站，從而提供盡可能高精度的真正的低氧培養(yǎng)。，氧氣對(duì)細(xì)胞的有效性是“看"氧分壓的值。氧分壓不僅隨著氧氣濃度的變化而改變，而且還與海拔和天氣相關(guān)。 HypoxyLab是真正的革命性產(chǎn)品，它直接調(diào)節(jié)工作站內(nèi)氧氣分壓的來提供低氧環(huán)境。我們用傳感器來測(cè)量氧氣而不補(bǔ)償濃度百分比來就可以表示低氧。

HypoxyLab以mmHg或kPa為單位表達(dá)工作站內(nèi)的氧環(huán)境，而對(duì)變化的氣候和海拔條件不敏感。這一*的科學(xué)方法使得HypoxyLab的準(zhǔn)確性增強(qiáng)，相比其他的設(shè)備高出30％以上，這依賴于我們的其他補(bǔ)償技術(shù)。由此，無論您的實(shí)驗(yàn)室位于世界何地，都能夠確保提供精確的低氧環(huán)境。

測(cè)量的金標(biāo)準(zhǔn)

對(duì)于那些要求原位氧測(cè)量“金標(biāo)準(zhǔn)"到細(xì)胞生長(zhǎng)的精確位置或所在層，HypoxyLab提供了一個(gè)可選的內(nèi)置 OxyLite™ 模塊來直接實(shí)時(shí)的進(jìn)行細(xì)胞培養(yǎng)介質(zhì)中的溶解氧測(cè)量。HypoxyLab具有工作站專用OxyLite傳感器連接器。當(dāng)連接一個(gè)OxyLite 探頭，定位在培養(yǎng)基中，其溶解氧精確濃度值會(huì)自動(dòng)地顯示在HypoxyLab的觸摸屏上并記錄在存儲(chǔ)卡中，顯示屏同時(shí)顯示的還有工作站的氧氣分壓，二氧化碳，溫度和濕度?？梢苑奖愕匕堰@些數(shù)據(jù)以LabChart™兼容格式記錄到到USB閃存盤中，可用來做進(jìn)一步的離線分析. 

真正的低氧復(fù)制

直接從細(xì)胞培養(yǎng)基中檢測(cè)氧氣

污染可控制的環(huán)境

的精度

輕便的上蓋

簡(jiǎn)潔大方的臺(tái)式外觀設(shè)計(jì)

規(guī)范采用氧分壓來衡量真正的低氧模擬在體氧環(huán)境

快速到達(dá)的低氧環(huán)境以及極底的氣體消耗

HEPA過濾標(biāo)準(zhǔn)，可隨意更換，操作簡(jiǎn)易

符合人體工程學(xué)設(shè)計(jì)

直觀便捷的觸摸屏

可選模塊 OxyLite™ 可檢測(cè)培養(yǎng)液內(nèi)部任意點(diǎn)位的溶解氧

可選微型電子顯微鏡

(Updated: June 2021)

Rapid Evaluation of Novel Therapeutic Strategies Using a 3D Collagen-Based Tissue-Like Model. Maury P, Porcel E, Mau A, Lux F, Tillement O, Mahou P, Schanne-Klein MC, and Lacombe S (2021). Front Bioeng Biotechnol. 2021 Feb 16;9:574035. doi: 10.3389/fbioe.2021.574035. eCollection 2021

Differentiated cells in prolonged hypoxia produce highly infectious native-like hepatitis C virus particles (2021). Cochard J, Bull-Maurer A, Tauber C, Burlaud-Gaillard J, Mazurier F, Meunier J-C, Roingeard P and Chouteau P. Hepatology. 2021 Mar 4. doi: 10.1002/hep.31788. Online ahead of print

Impact of the acidic environment on gene expression and functional parameters of tumors in vitro and in vivo (2021). Rauschner M, Lange L, Hüsing T, Reime S, Nolze A, Maschek M, Thews O & Riemann A. J Exp Clin Cancer Res, 40:10

SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury (2020). Lu B, et. al. Oxid Med Cell Longev. 2020 Oct 23;2020:2048210

Hypoxia Drives Dihydropyrimidine Dehydrogenase Expression in Macrophages and Confers Chemoresistance in Colorectal Cancer (2020). Malier M, Court M, Gharzeddine K, Laverierre M-H, Marsili S, Thomas F, Decaens T, Roth G & Millet A. /content/10.1101/2020.10.15.341123v1

Lactate preconditioning promotes a HIF-1α-mediated metabolic shift from OXPHOS to glycolysis in normal human diploid fibroblasts (2020). Kozlov AM, Lone A, Betts DH & Cumming RC. Nature Scientific Reports 10, 8388

Hypercapnia potentiates HIF-1α activation in the brain of rats exposed to intermittent hypoxia (2020). Tregubab PP, Malinovskayaa NA, Morguna AV, Osipovaa ED, Kulikovb VP, Kuzovkova DA, and Kovzelevc PD. J. Resp 278, 103442

Inhibition of Carbonic Anhydrase IX by Ureidosulfonamide Inhibitor U104 Reduces Prostate Cancer Cell Growth.... (2019). Riemann A, Güttler A, Haupt V, Wichmann H, Reime S, Bache M, Vordermark D and Thews O. Oncology Research

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment (2019). Court M, Malier M and Millet A. J. Vis. Exp.

The effect of hypoxia on ZEB1 expression in a mimetic system of the blood-brain barrier (2018). Leduc-Galindo D, Qvist P, Tóth AE, Frylａnd T, Nielsen MS, B?rglum AD and Christensen JH. Microvascular Research

Acidic extracellular environment affects miRNA expression in tumors in vitro and in vivo (2018). Riemann A, Reime S, and Thews O. Int J Cancer

Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro (2018). Gagner JP, Lechpammer M and Zagzag D. Methods Mol Biol.

Tumor Acidosis and Hypoxia Differently Modulate the Inflammatory Program: Measurements In Vitro and In Vivo (2017). Riemann A, Reime S and Thews O. Neoplasia

Glucose Metabolism and Oxygen Availability Govern Reactivation of the Latent Human Retrovirus HTLV-1 (2017). Kulkarni A, Mateus M, Thinnes CC, McCullagh JS, Schofield CJ, Taylor GP and Bangham CRM. Cell Chemical Biology