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佛波醇12-十四酸酯13-乙酸酯

注：本產(chǎn)品僅用于科研，不可用于臨床

生物活性

Phorbol 12-myristate 13-acetate (PMA), a phorbol ester, is a dual SphK and protein kinase C (PKC) activator^[1][2]. Phorbol 12-myristate 13-acetate is a NF-κB activator. Phorbol 12-myristate 13-acetate induces differentiation in THP-1 cells

IC₅₀ & Target

體外研究
(In Vitro)

In order to examine the role of PKC in p38MAPK phosphorylation, the cells are stimulated with the PKC activator, PMA (100 nM), which mimics the binding of DAG, the natural activator of PKC, to the C1 region of the PKCs. p38MAPK phosphorylation by PMA is observed in the two cell types similar to that observed by GnRH in αT3-1 cells, that is, a slow sustained activation (3.2-fold and 3.6-fold, respectively at 30 min). The paradoxical findings that PKCs activated by GnRH and PMA play a differential role in p38MAPK phosphorylation may be explained by differential localization of the PKCs. Basal, GnRH- and PMA- stimulation of p38MAPK phosphorylation in αT3-1 cells is mediated by Ca²⁺ influx via voltage-gated Ca²⁺ channels and Ca²⁺ mobilization, while in the differentiated LβT2 gonadotrope cells it is mediated only by Ca²⁺ mobilization^[2].
THP-1 cells are differentiated into macrophage-like cells (THP-1 macrophages) by incubation in the presence of PMA (200 ng/mL; 1-5 days), which leads to a macrophage-like phenotype characterized by changes in morphology and increased cell surface expression of CD11 and CD14^[3].
In the monocytic cell line THP-1, PMA results in a more differentiated phenotype than VD3, according to adherence, loss of proliferation, phagocytosis of latex beads, and expression of CD11b and CD14^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

PMA is a PKC agonist, which reverses the damage induced by 5-hydroxydecanoic acid (5-HD). Thus, activation of the mitoKATP protected mitochondrial function in SOD and MDA via the PKC pathway^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

616.83

性狀

Solid

Formula

C₃₆H₅₆O₈

中文名稱(chēng)

佛波醇12-十四酸酯13-乙酸酯；(12-)十四酸佛波酯(-13-)乙酸鹽；佛波酯

溶解性數(shù)據(jù)