只需 5 分鐘，ExoSAP-IT™ Express PCR Product Cleanup Reagent 即可從 PCR 反應(yīng)中除去過(guò)量的引物和未結(jié)合的核苷酸。與替代純化方法（如離心柱或微珠）相比，該酶法純化方法是一種更精確、產(chǎn)量更高和周轉(zhuǎn)時(shí)間更快的方法。
• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 99% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup
ExoSAP-IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer. 反應(yīng)設(shè)置僅需一個(gè)移液步驟即可完成，其后是兩次短暫孵育。次孵育可以水解過(guò)量的引物并使核苷酸去磷酸化。第二次高溫孵育在約一分鐘內(nèi)不可逆地滅活酶。將 ExoSAP-IT Express 試劑直接加入 PCR 產(chǎn)物中，可省略向管、孔或柱的轉(zhuǎn)移步驟并節(jié)省 PCR 擴(kuò)增子，從而有助于降低交叉污染的可能性；此外，也無(wú)需進(jìn)一步處理。樣品即可用于 DNA 測(cè)序或 SNP 分析。
• Remove unincorporated primers and nucleotides in about 5 minutes
• Prepare DNA sequencing samples with one pipetting step
• Conserve precious PCR products with up to 99% recovery, regardless of amplicon length
• Minimize spin column or bead cleanup
ExoSAP-IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes: a novel exonuclease I and shrimp alkaline phosphatase (SAP), in a specially formulated buffer. 反應(yīng)設(shè)置僅需一個(gè)移液步驟即可完成，其后是兩次短暫孵育。次孵育可以水解過(guò)量的引物并使核苷酸去磷酸化。第二次高溫孵育在約一分鐘內(nèi)不可逆地滅活酶。將 ExoSAP-IT Express 試劑直接加入 PCR 產(chǎn)物中，可省略向管、孔或柱的轉(zhuǎn)移步驟并節(jié)省 PCR 擴(kuò)增子，從而有助于降低交叉污染的可能性；此外，也無(wú)需進(jìn)一步處理。樣品即可用于 DNA 測(cè)序或 SNP 分析。
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