基質(zhì)金屬蛋白酶9(MMP9)單克隆抗體
Monoclonal Antibody to Matrix Metalloproteinase 9 (MMP9)
GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase
編號(hào)MAA553Hu22
物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
來(lái)源單抗制備
宿主小鼠
效價(jià)(克隆號(hào))16#
Ig亞型IgG2b Kappa
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無(wú)標(biāo)記物
免疫原RPA553Hu01-基質(zhì)金屬蛋白酶9(MMP9)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度1mg/ml
且適物種-
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
規(guī)格20µl100µl 200µl 1ml 10ml
價(jià)格￥ 1218
特異性
該抗體是針對(duì)MMP9的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別MMP9。
用法
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有明顯的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Bone and Joint Surgery | Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice [PubMed: 21119180] |
| Journal of Surgical Research | Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia [ScienceDirect: S0022480409001942] |
| Journal of Proteome Research | The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9 [ACS: pr2006395] |
| Zahedan Journal of Research in Medical Sciences | The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men [Zjrms: Source] |
| Experimental & Translational Stroke Medicine | Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke [PubMed: 21679435] |
| PLoS One | Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 [PubMed: PMC3797697] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| Rheumatology International | Hypothermia induced by adenosine 5′-monophosphate attenuates early stage injury in an acute gouty arthritis rat model [Pubmed: 23408150] |
| Clinical and Investigative Medicine | The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells [Pubmed: 23544611] |
| Acta Histochemica | RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells [Pubmed: 24681113] |
| International journal of colorectal disease | Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair [Pubmed: 23619615] |
| Journal of Periodontology | Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical Trial [Joponline: 130464] |
| Polski Przeglad Chirurgiczny | Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course [Pubmed: 23585206] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Int J Clin Exp Med. | Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model [Pubmed:Pmc4132129] |
| The Scientific World Journal | Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats [Pubmed:24701154] |
| Journal of Perinatology | Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion [Pubmed:25078863] |
| World J Emerg Med | Changes in biological behaviors of rat dermal fibroblasts induced by high expression of MMP9 [Pubmed:25215164] |
| PLOS ONE | Melissa officinalis Extract Inhibits Laser-Induced Choroidal Neovascularization in a Rat Model [Pubmed:25314292] |
| Stem Cells International | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro [Pubmed:25767513] |
| Physiology and Pharmacology | A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men [Phypha:Source] |
| Mediators of Inflammation | Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse [Journals: Mi] |
| Journal of Biomaterials and Tissue Engineering | MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 [Content: Asp] |
| PLOS | Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat [PubMed: 25945502] |
| Biomedical Materials | In vivo [PubMed: 25634132] |
| Histol Histopathol | Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells [PubMed: 26254457] |
| Int J Clin Exp Pathol | Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways [PubMed: 26191138] |
| Hattori and Ishihara Stem Cell Research & Therapy | Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells [PubMed: 25884474] |
| Neuropeptides | Melanocortin MC5 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat [PubMed: 25616531] |
| Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140] |
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