運(yùn)用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時(shí)對某個(gè)信號(hào)通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測,芯片上的基因包括了與研究對象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對不同的信號(hào)通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號(hào) | 名稱 | 基因列表 | 名稱 |
啟因生物(wcgene.com) | HUMAN-WC118 | Retinoic Acid Receptor Targets qpcr array | ADH1A, APOA2, BHLHE40, CD38, CDX1, CRABP1, CRABP2, CYP26A1, CYP26B1, CYP26C1, DHRS9, EFNB1, EGR1,EPO, FOXA1, GBX2, HNF1B, HOXA1, HOXA5, HOXB1, HOXB4, HSD17B2, KLF4, LEFTY1, MEIS2, MYC, PLAT, RARA,RARB, RARG, RARRES3, RBP1, RBP4, SHH, SREBF1, STRA6, STRA8, TFAP2C, TGM2, UCP1, WNT8A. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC118 | Retinoic Acid Receptor Targets qpcr array | Adh1a, Apoa2, Bhlhe40, Cd38, Cdx1, Crabp1, Crabp2, Cyp26a1, Cyp26b1, Cyp26c1, Dhrs9, Efnb1, Egr1, Epo,Foxa1, Gbx2, Hnf1b, Hoxa1, Hoxa5, Hoxb1, Hoxb4, Hsd17b2, Klf4, Lefty1, Meis2, Myc, Plat, Rara, Rarb, Rarg,Rbp1, Rbp4, Shh, Srebf1, Stra6, Stra8, Tfap2c, Tgm2, Ucp1, Wnt8a. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC123 | Retinoic Acid Receptor Targets qpcr array | Adh1a, Apoa2, Bhlhe40, Cd38, Cdkn2b, Cited2, Crabp1, Crabp2, Cyp26a1, Cyp26b1, Cyp26c1, Dhrs9, Efnb1,Egr1, Epo, Foxa1, Gbx2, Hnf1b, Hoxa1, Hoxa5, Hoxb1, Hoxb4, Hsd17b2, Klf4, Lefty1, Meis2, Myc, Plat, Rara,Rarb, Rbp1, Rbp4, Serpinb2, Shh, Srebf1, Stra6, Tfap2c, Tgm2, Ucp1, Wnt8a. | 300元/樣 |
檢測流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測,檢測RNA純度及完整度,提供RNA質(zhì)量報(bào)告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實(shí)時(shí)定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計(jì)算PCR芯片中的各個(gè)Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。