運(yùn)用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時(shí)對(duì)某個(gè)信號(hào)通路或疾病相關(guān)基因的多個(gè)基因的表達(dá)量變化進(jìn)行檢測(cè),芯片上的基因包括了與研究對(duì)象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對(duì)不同的信號(hào)通路和疾病相關(guān)基因設(shè)計(jì)了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個(gè)領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號(hào) | 名稱 | 基因列表 | 價(jià)格 |
啟因生物(wcgene.com) | HUMAN-WC095 | DNA Damage & Repair qpcr array | APEX1, ATM, ATR, BRCA1, BRCA2, CDKN1A (p21CIP1/WAF1), CHEK1, CHEK2 (RAD53), DDIT3 (GADD153/CHOP), ERCC1, ERCC2 (XPD), ERCC3 (XPB),ERCC5, ERCC6, GADD45A, LIG4, MDM2, MGMT (AGT), MLH1, MSH2, OGG1, PARP1 (ADPRT1), PCNA, PRKDC, RAD51, TP53, XPA, XPC, XRCC1, XRCC5. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC094 | DNA Damage & Repair qpcr array | Apex1, Atm, Brca1, Brca2, Cdkn1a (p21Cip1/Waf1), Chek1, Chek2 (Rad53), Ddit3 (Gadd153/Chop), Ercc1, Ercc2 (Xpd), Ercc3 (Xpb), Ercc5,Ercc6, Gadd45a, Lig4, Mdm2, Mgmt (Agt), Mlh1, Msh2, Ogg1, Parp1 (Adprt1), Pcna, Prkdc, Rad51, Trp53, Xpa, Xpc, Xrcc1, Xrcc5. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC096 | DNA Damage & Repair qpcr array | Apex1, Atm, Brca1, Brca2, Cdkn1a (p21Cip1/Waf1), Chek1, Chek2 (Rad53), Ddit3 (Gadd153/Chop), Ercc1, Ercc2 (Xpd), Ercc3 (Xpb), Ercc5,Ercc6, Gadd45a, Lig4, Mdm2, Mgmt (Agt), Mlh1, Msh2, Nbn (Nbs1), Ogg1, Parp1 (Adprt1), Pcna, Prkdc, Rad51, Tp53, Xpc, Xrcc1, Xrcc5. | 300元/樣 |
檢測(cè)流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測(cè),檢測(cè)RNA純度及完整度,提供RNA質(zhì)量報(bào)告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實(shí)時(shí)定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計(jì)算PCR芯片中的各個(gè)Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。