運用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時對某個信號通路或疾病相關(guān)基因的多個基因的表達(dá)量變化進(jìn)行檢測,芯片上的基因包括了與研究對象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對不同的信號通路和疾病相關(guān)基因設(shè)計了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號 | 名稱 | 基因列表 | 價格 |
啟因生物(wcgene.com) | HUMAN-WC074 | Other Regulators of Protein Folding qpcr array | ADCK3 (CABC1), ATF6, BAG1, BAG2, BAG3, BAG4, BAG5, CCS, CCT2, CCT3, CCT4, CCT5, CCT6A, CCT6B,CCT7, CRYAA, CRYAB, HSF1 (TCF5), HSF2, HSF4, HSPH1 (HSP105), PFDN1, PFDN2, SIL1, TCP1, TOR1A. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC073 | Other Regulators of Protein Folding qpcr array | Atf6, Bag1, Bag2, Bag3, Bag4, Bag5, Adck3, Ccs, Cct2, Cct3, Cct4, Cct5, Cct6a,Cct6b, Cct7, Cryaa, Cryab, Hsf1, Hsf2, Hsf4, Hsph1, Pfdn1, Pfdn2, Sil1, Tcp1, Tor1a. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC076 | Other Regulators of Protein Folding qpcr array | Atf6, Bag1, Bag3, Bag4, Bag5, Adck3, Ccs, Cct2, Cct3, Cct4, Cct5, Cct6a, Cct7, Cct8,Cryaa, Cryab, Tor1a, Hsf1 (Tcf5), Hsf2, Hsf4, Hsph1 (Hsp105), Pfdn1, Pfdn5, Pfdn6, Sil1, Tcp1. | 300元/樣 |
檢測流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測,檢測RNA純度及完整度,提供RNA質(zhì)量報告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實時定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計算PCR芯片中的各個Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。